F'Cm Plasmid of Escherichia coli
نویسندگان
چکیده
A strain of Pasteurella pestis, harboring the F'Cm plasmid from Escherichia coli, was able to donate its chromosome to auxotrophic recipient strains of P. pestis. The frequency of gene transfer in P. pestis was approximately 10-6 per donor cell, 100 times less efficient than gene transfer in Pasteurella pseudotuberculosis, but efficient enough to determine entry times for the markers histidine, threonine, and tryptophan and to show linkage to the markers arginine and pigmentation. An attempt to extend the conjugation system to different serotypes of P. pseudotuberculosis and to Yersinia enterocolitica did not succeed.
منابع مشابه
Gene transfer in Pasteurella pestis harboring the F'Cm plasmid of Escherichia coli.
A strain of Pasteurella pestis, harboring the F'Cm plasmid from Escherichia coli, was able to donate its chromosome to auxotrophic recipient strains of P. pestis. The frequency of gene transfer in P. pestis was approximately 10(-6) per donor cell, 100 times less efficient than gene transfer in Pasteurella pseudotuberculosis, but efficient enough to determine entry times for the markers histidin...
متن کاملStudy of antimicrobial susceptibility and plasmid analysis of Escherichia coli in Iran, Urmia
متن کامل
PCR Detection of Plasmid Mediated TEM, SHV and AmpC β-Lactamases in Community and Nosocomial Urinary Isolates of Escherichia coli
متن کامل
Investigation of adherent-invasive E. coli in patients with Crohn\'s disease
Background: Crohn's disease and Ulcerative colitis are known as inflammatory bowel disease with high morbidity which are as a result of increasing immune responses to intestinal microbiota in genetically susceptible individuals. The association of adherent invasive Escherichia coli with Crohn's disease in human has been discussed for decades. The principal aim of this study was to assess the re...
متن کاملGrowth of male-specific bacteriophage in Pasteurella harboring F-genotes derived from Escherichia coli.
When either the F' lac or the F'Cm plasmid was transferred from Escherichia coli into Pasteurella pseudotuberculosis, the P. pseudotuberculosis (F') strains isolated formed plaques with both ribonucleic acid (RNA)-containing and deoxyribonucleic acid-containing male-specific phages. In contrast, strains of P. pestis harboring E. coli (F') plasmids did not form plaques with male-specific phages,...
متن کامل